Journal: The Journal of Cell Biology

Article Title: Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth

doi: 10.1083/jcb.200212046

Figure Lengend Snippet: Gene expression by mpc at days 7 and 14 of culture

Article Snippet: MCP-1 (Beckman Coulter), MDC (R&D Systems), and VEGF (Cytimmune Sciences Inc.) concentrations in mpc-conditioned medium were determined by ELISA.

Techniques: Gene Expression

Journal: The Journal of Cell Biology

Article Title: Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth

doi: 10.1083/jcb.200212046

Figure Lengend Snippet: mpc constitutively express 5 monocyte chemotactic factors. (A) RT-PCR analysis of mpc mRNA at day 14 of FKN, MDC, MCP-1, VEGF, and β2M (β2microglobulin). (B and C) ELISA for (B) MDC, MCP-1, and (C) VEGF of mpc supernatants. Each symbol represents one culture estimated in triplicate. (D–G) Immunolabeling of (D) FKN, (E) MDC, (F) MCP-1, (G) VEGF using FITC-conjugated secondary antibody. Blue, DAPI stain.

Article Snippet: MCP-1 (Beckman Coulter), MDC (R&D Systems), and VEGF (Cytimmune Sciences Inc.) concentrations in mpc-conditioned medium were determined by ELISA.

Techniques: Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunolabeling, Staining

Journal: The Journal of Cell Biology

Article Title: Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth

doi: 10.1083/jcb.200212046

Figure Lengend Snippet: Five chemotactic systems ensure monocyte chemotaxis by mpc. Monocyte chemotaxis toward mpc-conditioned medium (day 14) was performed in the absence or presence of whole mice and rabbit IgGs or antibodies directed against MCP-1, MDC, VEGF, FKN, CX 3 CR1, uPAR, and uPA. Results are the means ± SEM of three experiments run in triplicate.

Article Snippet: MCP-1 (Beckman Coulter), MDC (R&D Systems), and VEGF (Cytimmune Sciences Inc.) concentrations in mpc-conditioned medium were determined by ELISA.

Techniques: Chemotaxis Assay

Journal: Scientific Reports

Article Title: Presence of periodontitis may synergistically contribute to cancer progression via Treg and IL-6

doi: 10.1038/s41598-022-15690-w

Figure Lengend Snippet: Results of the univariate analysis.

Article Snippet: Peripheral blood mononuclear cells (PBMC) obtained from the former were used for a flow cytometry (FCM) analysis and serum from the latter to analysis IL-6, VEGF, TGF-β1, and CCL22 concentrations measured by SRL Ltd. (Tokyo, Japan) and LSI Medience Corporation Ltd. (Tokyo, Japan).

Techniques:

Journal: bioRxiv

Article Title: Endometrial decidualization status modulates endometrial perivascular complexity and trophoblast outgrowth in gelatin hydrogels

doi: 10.1101/2022.11.08.515680

Figure Lengend Snippet: Optimization of an artificial endometrial perivascular niche. (A) Experimental summary. (B) Quantification of total vessel length per mm 3 , (C) total number of branches, (D) average branch length, and (E) total number of vessels for control and vascular endothelial growth factor (VEGF) samples (n=6 hydrogels per condition; 3 ROI imaged per gel and averaged) of varying endothelial to stromal cell ratios. Groups with different letters are statistically significantly different from each other. Data presented as mean ± standard deviation. Created with Biorender.com .

Article Snippet: The endogenous VEGF concentration in ECM was reported to be 2 ng/mL by the vendor (ScienCell).

Techniques: Control, Standard Deviation

Journal: Biomicrofluidics

Article Title: A microfluidic mammary gland coculture model using parallel 3D lumens for studying epithelial-endothelial migration in breast cancer

doi: 10.1063/1.5123912

Figure Lengend Snippet: Endothelial cell migration induced by VEGF gradient. (a) HUVECs in the left lumen were prestained with CellTracker Green to track cell migration toward the right lumen, which contained either a VEGF source or serum. Three representative images are shown, including serum-free media (VEGF 0), 500 ng/ml VEGF, and serum-containing medium (serum). (b) Graph of number of migrated cells within a single migration port vs VEGF concentration. (c) Graph of area coverage by migrated cells within a single migration port vs VEGF concentration. (d) Travel distances of individual migrated cells within a single migration port vs VEGF concentration, compiled from all experiments (n = 8). (e)–(f) COMSOL simulations of VEGF concentration profiles in the migration port showing (e) the case of 500 ng/ml starting VEGF concentration in the right channel (RC) at different timepoints, and (f) for t = 24 h for different starting VEGF concentrations in the RC. Note that the red curve in both graphs represents the same case, i.e., 24-h at 500 ng/ml starting VEGF concentration. ***p < 0.0001, **p < 0.01, and *p < 0.05.

Article Snippet: We performed numerical simulations in our 3D model over 24-h using COMSOL and studied the VEGF diffusion over time for a given starting VEGF concentration [e.g., 500 ng/ml starting VEGF concentration in ], and studied VEGF concentration profiles at 24 h for a range of different starting VEGF concentrations [ ].

Techniques: Migration, Concentration Assay